Journal: Communications Biology

Article Title: Slow viral propagation during initial phase of infection leads to viral persistence in mice

doi: 10.1038/s42003-021-02028-x

Figure Lengend Snippet: a , b C57BL/6 mice were infected with 2 × 10 4 pfu LCMV-WE (WE), and LCMV-Docile (Docile). At day 2 post-infection, spleen tissues were stained for LCMV antigen NP, CD169, CD11c, and B220 ( a ) or CD90.2, F4/80, and Ly6c ( b ) (representative sections are shown ( n = 4) scalebar = 20 µm). C57BL/6 mice were infected with LCMV-WE (WE), LCMV-Docile (Docile), or WE together with Docile (WE + Docile). c , d At day 2 post-infection, numbers of c splenic cDC (CD11c + MHC-II + ) and pDC (B220 + Siglec-H + ) were determined ( n = 9, gating strategy is shown in Supplementary Fig. ). d Co-stimulatory molecules CD40, CD80, and CD86 were measured on splenic cDC ( n = 6). e Serum IFN-α concentrations were determined at the indicated time post-infection ( n = 9). f Serum IL-6, g serum TNF-α, h serum IL-1β concentrations were measured at the indicated timepoints ( n = 5). (Error bars show SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, and ns indicates statistically not significant between the indicated groups).

Article Snippet: Antibodies against CD169 (Bio-Rad), CD11c, B220, CD90.2, F4/80, Ly6C (ebioscience), donkey-anti-rat secondary antibody (jackson immunoresearch), and self-made anti-LCMV monoclonal antibody (Clone: VL-4), were used.

Techniques: Infection, Staining

Journal: PLoS ONE

Article Title: Contrasting Roles of Islet Resident Immunoregulatory Macrophages and Dendritic Cells in Experimental Autoimmune Type 1 Diabetes

doi: 10.1371/journal.pone.0150792

Figure Lengend Snippet: Frozen pancreas sections from C57BL/6 mice (n = 5) were stained with a triple antibody cocktail reactive to glucagon, somatostatin, and pancreatic polypeptide (Light Blue); anti-TIM-4 (Green); anti-CD169 (Red); and Hoechst (Blue). White arrows indicate CD169+TIM-4+ cells. Individual panels are shown in . Photomicrographs were taken using 40x objective magnification. The bottom panel shows an enlarged area of the photomicrograph in the top panel.

Article Snippet: Pancreases were snap-frozen; stained with rat anti-mouse CD169, goat anti-mouse TIM-4 (R&D Systems, Minneapolis, MN) and a triple antibody cocktail containing rabbit anti-human glucagon, somatostatin, and pancreatic polypeptide.

Techniques: Staining

Journal: PLoS ONE

Article Title: Contrasting Roles of Islet Resident Immunoregulatory Macrophages and Dendritic Cells in Experimental Autoimmune Type 1 Diabetes

doi: 10.1371/journal.pone.0150792

Figure Lengend Snippet: Purified C57BL/6 pancreatic islets were enzymatically digested and stained with anti-CD45, anti-CD11c, anti-Ly6C, anti-F4/80, and anti-CD16/32. (A) Identification of live CD45+ (not shown) CD11c+Ly6C- (left panel) cells that exhibit an islet-resident macrophage (IRM, F4/80+CD16/32+) or DC (IRDC, F4/80-CD16/32-) phenotype (right panel) are shown. (B) FACS-sorted IRMs and IRDCs were analyzed for Csf1r transcript levels by real-time PCR. Data from independent experiments (n = 4 per group) and their mean are plotted; groups were compared with an unpaired t test. (C) Histograms show protein expression of CD169, TIM-4, and CX3CR1 on gated IRM (solid line) and IRDC (dashed line) subsets. Shaded histograms are the fluorescence minus one (FMO) controls. Geometric mean fluorescence intensities (MFIs) are indicated parenthetically. Data are representative of three independent experiments.

Article Snippet: Pancreases were snap-frozen; stained with rat anti-mouse CD169, goat anti-mouse TIM-4 (R&D Systems, Minneapolis, MN) and a triple antibody cocktail containing rabbit anti-human glucagon, somatostatin, and pancreatic polypeptide.

Techniques: Purification, Staining, Real-time Polymerase Chain Reaction, Expressing, Fluorescence